Generator

Part:BBa_K346037:Design

Designed by: miao jing   Group: iGEM10_Peking   (2010-10-15)


T3 promoter drived amplifier+Agn43


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 709
    Illegal NgoMIV site found at 1933
    Illegal NgoMIV site found at 2443
    Illegal NgoMIV site found at 2464
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 2203
    Illegal AgeI site found at 2617
    Illegal AgeI site found at 2859
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This amplifier part is designed by constructing PhiR73 and Po promoter upstream the Agn gene


Source

antigen 43 is cloned from K12 strain of E.coli

References